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Talk About The Effect Of Using Fermentation Broth Separation Opportunities

Talk About The Effect Of Using Fermentation Broth Separation Opportunities

May 12, 2020

The fermentation broth separator is a mechanical filtration method. It is suitable for separating the tiny suspended substances present in the liquid to the greatest extent, to achieve the purpose of separating the solid and liquid phases. The difference between solid-liquid separation and other methods is that the gap of the filter medium is extremely small. With the help of the centrifugal force of the screen rotation, the fermentation liquid has a high flow rate and retains suspended solids under low hydraulic resistance.


The most important step in extracting biochemical substances from fermentation broth or cell culture broth of E. coli genetically engineered bacteria is pretreatment and solid-liquid separation. The purpose is not only to isolate E. coli genetically engineered cells and other suspended particles, but also to remove some soluble impurities and change the properties of the fermentation broth to facilitate subsequent physical and chemical extraction of metabolites from the clarified fermentation broth, or Starting from Escherichia coli genetically engineered bacteria, cell disruption, fragmentation and extraction of intracellular products are carried out. There are many impurities in the fermentation broth of genetically engineered bacteria of E. coli, some of which not only directly affect the quality and yield of the product, but also have a great influence on the subsequent extraction and refining. Therefore, in the pretreatment, these impurities must be precipitated by appropriate methods It is removed during solid-liquid separation to facilitate the subsequent extraction and purification process to proceed smoothly. Centrifugal separation is mainly used for solid-liquid separation of fermentation liquid of Escherichia coli genetic engineering bacteria. We use centrifugal force generated by high-speed rotation of the centrifuge to achieve solid-liquid separation of fermentation liquid. Etc.